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Role of Overwintering Forms of Erysiphe necator in Epidemiology of Grapevine Powdery Mildew in Palestinian Vineyards

Titre
Role of Overwintering Forms of Erysiphe necator in Epidemiology of Grapevine Powdery Mildew in Palestinian Vineyards
Résumé
Abstract The overwintering modes of E. necator were studied on Palestinian vineyards, through observations on the differentiation and maturation of cleistothecia and on the occurrence of flag-shoots (deriving from overwintering mycelium) in vineyards. Field surveys were carried out in 17 vineyards for the presence of Flag shoots and cleistothecia, both forms were not observed. Genetic structure and composition of E. necator populations were investigated by application of already available SCAR (Sequence Characterized Amplified Region) primers specific for the "flag-shoot" and "ascospore" biotypes. These primers were used to evaluate the dynamics of the spatial and temporal distribution of the two biotypes, into fungal populations present in 8 vineyards, with different cultivars and spray histories, in various Palestinian districts (Hebron, Bethlehem, Jerusalem, Ramallah, Jericho, Nablus, Jenin, Tulkarm). 397 samples were analyzed by the uses of the primer pairs UnE-UnF in PCR reactions, all were reveal to be of the ascospore biotypes. This finding confirm an early studies show that the “flag shoot” biotype, appears soon after bud breaking and disappears later, while the “ascospore” biotype is more frequently associated to later infections and bunches dameages. Such information would be helpful to understand the reasons underlying possible temporal evolution of the pathogen's populations in vineyards, and can have important implications for powdery mildew rationale control strategies.
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Année
2014
Publié en
Palestine Technical University Research Journal, 2 (1): 20-25
31/03/14 - Introduit par: Ruba Shawqi Abuamsha
Quantitative & qualitative enhancement of crop products
Thème principal: Quantitative & qualitative enhancement of crop products
Sous-catégorie Post-harvesting