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Setting up and evaluation of new primer sets for the detection of Spiroplasma citri the causal agent of Citrus Stubborn disease

Titre
Setting up and evaluation of new primer sets for the detection of Spiroplasma citri the causal agent of Citrus Stubborn disease
Résumé
Citrus stubborn disease caused by Spiroplasma citri is a limiting factor for citrus production in countries with desert or semi-arid conditions in the Mediterranean basin. In order to develop a PCR assay specific and sensitive for detecting Mediterranean S. citri strains, three new PCR primers were designed, on the basis of repetitive sequences annotated “Hypothetical proteins”; The primer pairs were evaluated for their sensitivity by PCR assay with serial dilutions of positive S. citri cultures and DNA extracted from S. citri infected plants, while the specificity was determined by PCR using the DNA extracted from cultures of other Spiroplasma species. Primer pairs were validated by comparing them with PCR primer pairs previously designed in other laboratories..
Plus
Année
2012
Publié en
International Citrus Congress
29/11/13 - Introduit par: Ahmed Abdelfattah
Quantitative & qualitative enhancement of crop products
Thème principal: Quantitative & qualitative enhancement of crop products
Sous-catégorie Pre-harvesting